This synthesized the recombinant gene by integrating the N-terminal 6xHis tag sequence into the targeted gene encoding the aa301-412 of the human TGFB3. The synthesized gene was subsequently cloned into an expression vector. After cloning, the expression vector was introduced into the E.coli for expression. The product was purified to obtain the recombinant human TGFB3 protein carrying N-terminal 6xHis-Tag. The SDS-PAGE assayed the purity of this recombinant TGFB3 protein greater than 90%. This TGFB3 protein migrated along the gel to a band of about 16kD molecular weight. TGFB3 is a gene encoding a protein named transforming growth factor beta-3 proprotein in human and belongs to TGF-beta family. Transforming growth factor beta-3 proprotein can be cleaved into two chains, including latency-associated peptide (LAP) and transforming growth factor beta-3 (TGF-beta-3). LAP is required to maintain the Transforming growth factor beta-3 (TGF-beta-3) chain in a latent state during storage in extracellular matrix. TGF-beta-3 is known as cytokine and is involved in cell differentiation, embryogenesis and development, which is found throughout the body and is required for development before birth and throughout life. Source: Recombinant protein corresponding to aa304-413 from human Transforming growth factor beta-2, fused to 6X His-Tag at N-terminal, expressed in E.coli. Molecular Weight: ~16.7kD Amino Acid Sequence: ALDTNYCFRNLEENCCVRPLYIDFRQDLGWKWVHEPKGYYANFCSGPCPYLRSADTTHSTVLGLYNTLNPEASASPCCVPQDLEPLTILYYVGRTPKVEQLSNMVVKSCKCS Storage and Stability: Lyophilized and reconstituted products are stable for 6 months after receipt at -20C. Reconstitute with sterile ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Supplied as a lyophilized powder from 20mM Tris-HCl, 0.5M sodium chloride, pH 8.0, 6% trehalose. Reconstitute with sterile ddH2O to a concentration of 0.1-1mg/ml.
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