Troponin I, Recombinant, Astacus leptodactylus, aa1-201, His-Tag, Myc-Tag

Catalog Number: USB-586631
Article Name: Troponin I, Recombinant, Astacus leptodactylus, aa1-201, His-Tag, Myc-Tag
Biozol Catalog Number: USB-586631
Supplier Catalog Number: 586631
Alternative Catalog Number: USB-586631-20, USB-586631-100
Manufacturer: US Biological
Category: Molekularbiologie
Troponin I is the actomyosin ATPase inhibitory subunit present in the thin filament regulatory complex. Source: Recombinant protein corresponding to aa1-201 from Astacus leptodactylus Troponin I, fused to 10X His-Tag at N-terminal and Myc-Tag at C-terminal, expressed in E.coli. Molecular Weight: ~30.9kD Amino Acid Sequence: ADKAKAAEEAKKKQDDIDRKKAEVRKRLEEQSLKKQKKGFMTPERKKKLRLLLRKKAAEELKKEQERKAGERRKIIDQRCGQPKNLDGANEEQLRAIIKEYFDHTAQIESDKYDVELEIIRKDYEINELNIQVNDLRGKFIKPTLKKVSKYENKFAKLQKKAAEFNFRNQLKTVKKKEFELEDDKGATEGDGPAAEEVAAE Storage and Stability: Lyophilized and reconstituted products are stable for 6 months after receipt at -20C. Reconstitute with sterile ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Molecular Weight: 30.9
UniProt: P05547
Purity: 90% (SDS-PAGE)
Form: Supplied as a lyophilized powder from 20mM Tris-HCl, 0.5M sodium chloride, pH 8.0, 6% trehalose. Reconstitute with sterile ddH2O to a concentration of 0.1-1mg/ml.