Angiotensin-converting enzyme 2 (ACE2), also known as ACAH, is an essential counter-regulatory carboxypeptidase of the renin-angiotensin hormone system, which is a critical regulator of blood volume, systemic vascular resistance, and thus cardiovascular homeostasis. ACE2 is a suppressor of renin-angiotensin system (RAS). ACE2 converts angiotensin I to angiotensin 1-9, a nine-amino acid peptide with anti-hypertrophic effects in cardiomyocytes, and angiotensin II to angiotensin 1-7, a vasodilator. Besides the role in the cardiovascular system, ACE2 also plays a role in lung diseases as a receptor for SARS-CoV-2. Recently, with the COVID19 caused by SARS-CoV-2 spreads around the world, ACE2 functions as a receptor on the cell surface to bind with spike protein of SARS-CoV-2 in SARS-CoV-2 infection. And the mechanism of SARS-CoV-2 invasion via ACE2 are gradually being revealed. Based on these studies, there are more coronavirus-host interactome targets which have been found, such as TMPRSS2 and CD147. All of these works are critical to SARS-CoV-2 treatment, and provide potential targets of drug development for SARS-CoV-2 therapy. Partial recombinant protein corresponding to aa18-740 from human Angiotensin-converting enzyme 2, fused to 6X His-Tag at C-terminal, expressed in Mammalian cells. Accession/Uniprot: Q9BYF1 Molecular Weight: ~84.63kD Biological Activity: Immobilized Human ACE-2-His at 10ug/ml (100ul/well) can bind 2019-nCoV S Protein RBD-mFc. The ED50 of 2019-nCoV S Protein RBD-mFc is 19.97ng/ml. Amino Acid Sequence: QSTIEEQAKTFLDKFNHEAEDLFYQSSLASWNYNTNITEENVQNMNNAGDKWSAFLKEQSTLAQMYPLQEIQNLTVKLQLQALQQNGSSVLSEDKSKRLNTILNTMSTIYSTGKVCNPDNPQECLLLEPGLNEIMANSLDYNERLWAWESWRSEVGKQLRPLYEEYVVLKNEMARANHYEDYGDYWRGDYEVNGVDGYDYSRGQLIEDVEHTFEEIKPLYEHLHAYVRAKLMNAYPSYISPIGCLPAHLLGDMWGRFWTNLYSLTVPFGQKPNIDVTDAMVDQAWDAQRIFKEAEKFFVSVGLPNMTQGFWENSMLTDPGNVQKAVCHPTAWDLGKGDFRILMCTKVTMDDFLTAHHEMGHIQYDMAYAAQPFLLRNGANEGFHEAVGEIMSLSAATPKHLKSIGLLSPDFQEDNETEINFLLKQALTIVGTLPFTYMLEKWRWMVFKGEIPKDQWMKKWWEMKREIVGVVEPVPHDETYCDPASLFHVSNDYSFIRYYTRTLYQFQFQEALCQAAKHEGPLHKCDISNSTEAGQKLFNMLRLGKSEPWTLALENVVGAKNMNVRPLLNYFEPLFTWLKDQNKNSFVGWSTDWSPYADQSIKVRISLKSALGDKAYEWNDNEMYLFRSSVAYAMRQYFLKVKNQMILFGEEDVRVANLKPRISFNFFVTAPKNVSDIIPRTEVEKAIRMSRSRINDAFRLNDNSLEFLGIQPTLGPPNQPPVS Storage and Stability: Lyophilized and reconstituted products are stable for 12 months after receipt at -20C. Reconstitute with sterile ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Supplied as a lyophilized powder from 20mM Tris-HCl, pH 7.4, 300mM sodium chloride, 1mM ZnCl2, 10% glycerol. Reconstitute with sterile ddH2O, 0.1% BSA to a concentration of 0.1-1mg/ml.
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