Participates in at least several B-cell activation processes as well as of other cell types. Recombinant protein corresponding to aa25-153 from human Interleukin 4, expressed in E. coli. Molecular Weight: ~15.1kD Biological Activity: The ED50 as determined by the dose-dependent stimulation of TF-1 cells is less than 2 ng/ml. Specific activity is 5.0 x 10e6 IU/mg. Amino Acid Sequence: HKCDITLQEIIKTLNSLTEQKTLCTELTVTDIFAASKNTTEKETFCRAATVLRQFYSHHEKDTRCLGATAQQFHRHKQLIRFLKRLDRNLWGLAGLNSCPVKEANQSTLENFLERLKTIMREKYSKCSS Storage and Stability: Lyophilized and reconstituted products are stable for 6 months after receipt at -20C. Reconstitute with sterile ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
