Factor that induces terminal differentiation of late-developing B-cells to immunoglobulin secreting cells. Recombinant protein corresponding to aa20-134 from human Interleukin 5, fused to 6xHis-Tag at C-terminal, expressed in Mammalian cells. Molecular Weight: ~14.2kD Biological Activity: The ED50 as determined in a cell proliferation assay using TF?1 human erythroleukemic cells is less than 0.8ng/ml. Amino Acid Sequence: IPTEIPTSAL VKETLALLST HRTLLIANET LRIPVPVHKN HQLCTEEIFQ GIGTLESQTV QGGTVERLFK NLSLIKKYID GQKKKCGEER RRVNQFLDYL QEFLGVMNTE WIIES Storage and Stability: Lyophilized and reconstituted products are stable for 6 months after receipt at -20C. Reconstitute with sterile ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
