Membrane-anchored forms may play a role in cellular adhesion. Partial recombinant protein corresponding to aa296-580 from human Mesothelin, fused to hFc-Tag at C-terminal, expressed in Mammalian cells. Molecular Weight: ~60.4kD Biological Activity: 1. Measured by its binding ability in a functional ELISA. Immobilized MSLN at 2ug/ml can bind Anti-MSLN rabbit monoclonal antibody, the EC50 of the MSLN protein is 2.657-3.177ng/ml. 2. Measured by its binding ability in a functional ELISA. Immobilized MUC1 at 10ug/ml can bind MSLN, the EC50 is 460.7-662.2ng/ml. Amino Acid Sequence: EVEKTACPSGKKAREIDESLIFYKKWELEACVDAALLATQMDRVNAIPFTYEQLDVLKHKLDELYPQGYPESVIQHLGYLFLKMSPEDIRKWNVTSLETLKALLEVNKGHEMSPQVATLIDRFVKGRGQLDKDTLDTLTAFYPGYLCSLSPEELSSVPPSSIWAVRPQDLDTCDPRQLDVLYPKARLAFQNMNGSEYFVKIQSFLGGAPTEDLKALSQQNVSMDLATFMKLRTDAVLPLTVAEVQKLLGPHVEGLKAEERHRPVRDWILRQRQDDLDTLGLGLQG Storage and Stability: Lyophilized and reconstituted products are stable for 6 months after receipt at -20C. Reconstitute with sterile ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
