Recombinant protein corresponding to aa28-144 from rat Noggin, with two N-Terminal Tags, His-Tag and GST Tag expressed in E. coli. Uniprot/Acession: Q62809. Activity: NOG (Noggin) is a signaling molecule that plays an important role in promoting somite patterning in the developing embryo, essential for cartilage morphogenesis and joint formation. It is considered as an inhibitor in bone morphogenetic proteins (BMP) signaling, by binding with BMP4, thus a binding ELISA assay was conducted to detect the association of recombinant rat NOG with BMP4. Briefly, NOG were diluted serially in PBS with 0.01%BSA (pH 7.4). Duplicate samples of 100uL were then transferred to BMP4-coated microtiter wells and incubated for 2h at 37C. Wells were washed with PBST and incubated for 1h with anti-NOG pAb, then aspirated and washed 3 times. After incubation with HRP labelled secondary antibody, wells were aspirated and washed 3 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37C. Finally, add 50ul stop solution to the wells and read at 450nm immediately. The binding activity of NOG with BMP4 was shown in Figure 1 and this effect was in a dose dependent manner. Predicted Isoelectric Point: 10.0 Predicted Molecular Mass: 43.6kD Accurate Molecular Mass: 44kD as determined by SDS-PAGE reducing conditions. Amino Acid Sequence: MPS EIKGLEFSEG LAQGKKQRLS KKLRRKLQMW LWSQTFCPVL YAWNDLGSRF WARYVKVGSC FSKRSCSVPE GMVCKPSKSV HLTVLRWRCQ RRGGQRCGWI PIQYPIISEC KCSC Applications: Suitable for use in Cell Culture and Activity Assays. Other applications not tested. Recommended Dilutions: Optimal dilutions to be determined by the researcher. Storage and Stability: Lyophilized and reconstituted products are stable for 6 months after receipt at -70C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -70C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
