AatII

Product Overview

• Article Name: AatII
• Biozol Catalog Number: USB-A0002
• Supplier Catalog Number: A0002
• Alternative Catalog Number: USB-A0002-300,USB-A0002-1500
• Manufacturer: US Biological
• Category: Molekularbiologie

Product Description

Sequence: 5-G A C G T C-3 3-C T G C A G-5 Supplied With: Restriction Enzyme Buffer A (R1625): (for 100% AatII digestion) 33mM Tris-acetate (pH 7.9), 10mM magnesium acetate, 66mM potassium acetate and 0.1mg/ml BSA. Incubate at 37C. Diluent Buffer: 10mM Tris-HCl (pH 7.4 at 25C), 100mM potassium chloride, 1mM EDTA, 1mM DTT, 0.2mg/ml BSA and 50% glycerol. Storage Buffer: 10mM Tris-HCl (pH 7.4 at 25C), 100mM potassium chloride, 1mM DTT, 0.1mM EDTA, 0.2mg/ml BSA and 50% glycerol. Overdigestion Assay: No detectable change in the specific fragmentation pattern is observed after a 160-fold overdigestion with AatII (10u/ug lambda DNA x 16 hours). Ligation/Recutting Assay: After 50-fold overdigestion (3u/ug DNA x 17 hours) with AatII more than 95% of the digested DNA fragments can be ligated at a 5-termini concentration of 0.1uM. More than 95% of these sites can be recut. Labeled Oligonucleotide (LO) Assay: No detectable degradation of a single-stranded and double-stranded labeled oligonucleotide was observed after incubation with 10 units of restriction endonuclease for 4 hours. Storage and Stability: May be stored at 4C for short-term only. For long-term storage, aliquot and store at -20C. Aliquots are stable for at least 12 months at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.