Synthetic (linear) peptide (EAIYAAPFAKKK), biotinylated at the C-terminus. Substrate for Abl kinase. (with an extra K conjugated to Biotin) Application: Suitable for use in Abl kinase assays. Other applications not tested. Recommended Dilution: Use at 25-50uM final concentration for in vitro Abl kinase assays. 25uM was phosphorylated using 10-20ng of Abl, active. The substrate was captured using Streptavidin, agarose conjugate. Assay results show a 332-fold increase in phosphate transferred to the peptide over background. Optimal dilutions to be determined by the researcher. Storage and Stability: Lyophilized powder may be stored at 4C for short-term only. Stable for 12 months at -20C. Reconstitute to nominal volume (see reconstitution instructions for peptides) and store at -20C.
Molecular Weight:
1563
Purity:
~ 90%, as determined by HPLC
Form:
Supplied as a lyophilized powder.
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