Synthetic linear peptide (EAIYAAPFAKKK)(K), substrate for Abl kinase. Abl Protein Tyrosine Kinase (AbI) is a truncated form of the v-AbI Protein Tyrosine Kinase, a partner in the Gag-Abl fusion protein of the Abelson murine leukemia virus. Abl contains 407 amino acids (residues 237-643 of the p120-gag-abl polyprotein) which include the kinase catalytic domain, SH2 domain on the N-terminus and the I237M mutation. Abl is identical to the normal c-Abl. Abl is autophosphorylated on tyrosine(s). Applications: Suitable for use in Kinase assay. Other applications not tested. Recommended Dilution: Kinase Assay: This lot at 50uM, was successfully phosphorylated using Abl, active. Assay background was subtracted from the actual counts. Representative results from a previous lot are shown at right. This peptide may also be used as a substrate for Abl (T315I), active and Abl, active, mouse. Optimal dilutions to be determined by the researcher. Storage and Stability: Lyophilized powder may be stored at 4C for short-term only. Stable for 12 months at -20C. Reconstitute to nominal volume (see reconstitution instructions for peptides) and store at -20C. For maximum recovery of product, centrifuge the original vial prior to removing the cap. Further dilutions can be made in assay buffer.
Purity:
95+% HPLC, Mass Spec
Form:
Supplied as a white to off-white lyophilized powder.
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