Acinus (AAE7, ACN1, AC NON-UTILIZING 1, ACYL-ACTIVATING ENZYME 7, AT3G16910.1, Apoptotic Chromatin condensation inducer in nucleus) (Control Peptide)
Biozol Catalog Number:
USB-A0600-02P
Supplier Catalog Number:
A0600-02P
Alternative Catalog Number:
USB-A0600-02P-50
Manufacturer:
US Biological
Category:
Molekularbiologie
Peptide (DTSENRPENDVPEPP) corresponding to amino acids 775 to 789 of human AcinusL, 48 to 62 of human AcinusS, or 17 to 31 of human AcinusS, which differ from those of mouse Acinus by one amino acid (2). For use with cat. no. A0600-02. Chromatin condensation and nuclear fragmentation (CCNF) is the hallmark of apoptosis. CCNF is triggered by the activation of members of caspase family, caspase activated DNase (CAD/DFF40), and several novel proteins including AIF and CIDE (1). A new inducer of chromatin condensation was recently identified and designated Acinus (for apoptotic chromatin condensation inducer in the nucleus). Acinus is cleaved by caspase-3 and an additional unknown protease generating a small active peptide p17, which causes chromatin condensation in vitro when it is added to purified nuclei. Acinus also induces apoptotic chromatin condensation in cells. Acinus is ubiquitously expressed. Three different spliced forms of Acinus have been identified in human and mouse and designated AcinusL, AcinusS and AcinusS (2). Applications: Suitable for use as control peptide in ELISA and Western Blot. Other applications not tested. Positive Control: K562 cell lysate Storage and Stability: May be stored at 4C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and add glycerol (40-50%). Freeze at -20C or colder. Aliquots are stable for at least 6 months at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Purity:
Purified
Form:
Supplied as a liquid in PBS, pH 7.2, 0.1% BSA, 0.02% sodium azide.
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