Actin and myosin are the two major cytoskeleton proteins implicated in cellular movement, secretion, phagocytosis, and kinesis. Actin is one of the most conserved cellular protein. At least 6 actin isoforms have been identified by protein sequence analyses. Four actin isoforms represent the differentiation markers of muscle tissues. There are three a-actins: a-skeletal, a-cardiac, and a-smooth muscle), one b-actin (b-non-muscle), and two g-actins (g- smooth muscle and g-non-muscle). Actin isoform are >90% conserved, except in the N-terminal 18aa (50-60% homology). Beta-actin protein and mRNA levels are often used as a reference for comparing changes in cellular protein/mRNA levels by Western or Northern blots. Source: Synthetic peptide corresponding to N-terminal 15aa of human beta-actin, non-muscle. Applications: Suitable for use in Antibody Blocking and ELISA. Other applications not tested. Recommended Dilution: ELISA: 1ug/ml Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Purity:
Purified
Form:
Supplied as a lyophilized powder from PBS, 0.05% sodium azide.
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