Synthetic peptide corresponding to 14 amino acids near the carboxy-terminus of human AID (GenBank accession no. AAM95402). See corresponding antibody A0855-75J. Activation-induced cytidine deaminase (AID) was initially discovered as a homolog of the apolipoprotein B RNAediting cytidine deaminase 1 (APOBEC1) that showed cytidine deaminase properties in stimulated B cell lines (1). It is necessary for somatic hypermutation and class switch recombination in B cells (2), but inappropriate or dysregulated expression AID is often found in tumors and B cell neoplasms (2,3). Although it is structurally and functionally similar to the APOBEC proteins (reviewed in 4), it appears unlikely that AID deaminates dC to dU residues in HIV cDNA as does APOBEC3G (5). Applications: Suitable for use as a blocking peptide in ELISA, Western Blot with A0855-75J. Other applications not tested. Recommended Dilution: Western Blot: AID peptide is used for blocking the activity of AID antibody (Cat. No. A0855-75J). It usually blocks the antibody activity completely in Western blot by incubating the peptide with equal volume of antibody for 30 min at 37°C. Optimal dilutions to be determined by the researcher. Storage and Stability: Liquid peptide is stable for 12 months at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
