Source: HeLa cells, inoculated with Adenovirus, type 6, strain Tonsil 99. Preparation: Virus was grown on monolayer of HeLa cells. When the cultures showed pronounced c.p.e. virus containing medium was purified from cell fragments by low speed centrifugation (4,000 rpm 40 min.). Virus was pelleted by high speed centrifugation at 18,000 rpm for 2.5 hours in the SW27 rotor for 20 min. at 4C, then the supernatant was collected. Applications: Serological studies of influenza A virus, immunogen for antibody production. Immunological Activity: Tested with anti-adenovirus antibodies in ELISA. Inactivation: Thimerosal and beta-propiolactone Storage and Stability: May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for 6 months after receipt at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. Note: No test guarantees a product to be non-infectious. All materials should be handled as if potentially infectious. Generally accepted laboratory practices appropriate for infectious materials should be employed when handling this product.
Purity:
90% (SDS-PAGE)
Form:
Supplied as a liquid in STE, 0.005% thimerosal, 0.09% sodium azide.
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