Agarase, beta (beta-Agarase, Agarose 4-glycanohydrolase, E.C. 3.2.1.81), CAS [[37288-57-6]]

Product Overview

• Article Name: Agarase, beta (beta-Agarase, Agarose 4-glycanohydrolase, E.C. 3.2.1.81), CAS [[37288-57-6]]
• Biozol Catalog Number: USB-A0965
• Supplier Catalog Number: A0965
• Alternative Catalog Number: USB-A0965-1,USB-A0965-5
• Manufacturer: US Biological
• Category: Molekularbiologie

Product Description

beta-Agarase has a molecular weight of 32kD and hydrolyzes the 1,4-beta-D-galactosidic linkages in agarose, giving the tetramer as the predominant product. It may be used to investigate the structure of different galactans and as an enzymatic reagent for degrading agarose used as a gel medium in electrophoresis. This allows the isolation of oligonucleotides and DNA fragments from gels prepared with low melting point agarose. Synonyms: beta-Agarase, Agarose 4-glycanohydrolase, E.C. 3.2.1.81 CAS No: 37288-57-6 Source: Pseudomonas atlantica Appearance: Off-white to light yellow or light tan lyophilized powder Protein: 10-30% Activity (U/mgS) 0units/mg solid Specific Activity: 5,000U/mg protein Unit Definition: One unit will produce 1.0ug of reducing sugar (measured as D-galactose) from agar per minute at pH 6.0 and 40C. Applications: Quantitative recovery of DNA or RNA from low melting point agarose gels. The recovered nucleic acids can be subjected to many further manipulations such as restriction endonuclease digestion, ligation, labeling, sequencing, amplification, etc. Storage and Stability: Lyophilized powder may be stored at 4C. Stable for 6 months after receipt at 4C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological. Toxicity and Hazards: All products should be handled by qualified personnel only, trained in laboratory procedures.

Product Properties

• Purity: Specific Activity: 5,000U/mg protein
• Form: Off-white to light yellow or light tan lyophilized powder
• CAS Number: [37288-57-6]