AgRP, Recombinant, Human, Western Blot Positive Control (Agouti Related Protein, ART, AGRT, ASIP2, Agouti-related Transcript)

Product Overview

• Article Name: AgRP, Recombinant, Human, Western Blot Positive Control (Agouti Related Protein, ART, AGRT, ASIP2, Agouti-related Transcript)
• Biozol Catalog Number: USB-A1059-62P
• Supplier Catalog Number: A1059-62P
• Alternative Catalog Number: USB-A1059-62P-100
• Manufacturer: US Biological
• Category: Molekularbiologie
• Application: WB

Product Description

Several common diseases such as type II diabetes, hypertension, cardiovascular diseases, hyperlipidemia, and some cancers are associated with obesity. An abnormal increase in body fat relative to lean tissue mass has been used as an indicator of obesity. High fat diet, certain environmental factors, and genetic linkage are the primary causes of obesity. In order to understand the genetic basis of obesity, several monogenic murine obesity models have been characterized including obese (Ob), diabetes (db), fat (fat), agouti yellow (Ay), and tubby (tub). More recently, Tub, the human homolog of mouse Tub, TULP1 & TULP2 (for Tubby Like Proteins) and Agouti related protein (AGRP) have been cloned. The obesity associated with Ay mice may be due to ectopic expression of a secreted protein Agouti. Agouti protein (132aa in human) is normally expressed in skin but its ubiquitous expression causes obesity. Agouti is a paracrine-signaling molecule that affects pigmentation by inhibiting the melanocortin receptor 1 (MCR-1). However, recombinant Agouti protein also antagonizes the MC2R and MC4R. AGRP (132aa in human, chromosome 16q21) is normally expressed in adrenal and hypothalamus. AGRP levels are increased several folds in ob/ob mice. AGRP is a strong antagonist of MC3R and MC4R. Ubiquitous expression of AGRP in transgenic mice causes obesity without altering skin pigmentation. Source: Human AGRP, 132aa, expressed as his-tagged fusion protein in baculovirus cell line Sf21. Molecular Weight: ~14-19kD Applications: Suitable for use in Western Blot. Not suitable for use in ELISA. Other applications not tested. Recommended Dilution: Western Blot: 10ul/lane. SDS may crystallize in cold conditions. It should redissolve by warming before taking it from the stock. It should be heated once prior to loading on gels. If the product has been stored for several weeks, then it may be preferable to add 5ul of fresh 2x sample buffer per 10ul of the Western Blot solution prior to heating and loading on gels. Optimal dilutions to be determined by the researcher. Storage and Stability: Lyophilized powder may be stored at -20C. Stable for 12 months at -20C. Reconstitute with sterile buffer or ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Product Properties

• Molecular Weight: 1419
• Purity: Highly Purified (~95%)
• Form: Supplied as a lyophilized powder from PBS, 0.05% sodium azide.