Lectins are derived from the extracts of plants, animals, viruses and microorganisms and are known to agglutinate red blood cells. These agglutinins can select cell types according to blood group activities utilizing sugar-binding mechanisms. Lectins form precipitates with glycoconjugates and are useful for identifying or separating oligosaccharides with identical sugar compositions such as galactose, mannose or glucose. Pure Amaranthus caudatus lectin (ACA) from Tassel flower, Inca wheat, Biotin conjugated. Carbohydrate Specificity: Galactose beta (1,3) N-Acetylgalactosamine (the T antigen). Inhibitory Carbohydrate: Lactose>high concentrations of beta-Galactose. Activity: Less than 0.5ug/ml will agglutinate neuraminidase treated human red blood cells. Chemical Used for Conjugation: Biotinyl N-hydroxysuccinimide ester (BNOHSE). Storage and Stability: May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for 6 months after receipt at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Purity:
Purified
Form:
Supplied as a liquid in 0.01M PBS, pH 7.2-7.4. Labeled with Biotin.
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