Ammonia Assay Kit, BioAssay(TM)

Catalog Number: USB-A1383-56
Article Name: Ammonia Assay Kit, BioAssay(TM)
Biozol Catalog Number: USB-A1383-56
Supplier Catalog Number: A1383-56
Alternative Catalog Number: USB-A1383-56-1
Manufacturer: US Biological
Category: Kits/Assays
Ammonia (NH3) or its ion form ammonium (NH4+) is an important source of nitrogen for living systems and is ubiquitously present in the nature. Simple, direct and automation-ready procedures for measuring NH3 are very desirable. Ammonia/ammonium assay is based on an improved o-phthalaldehyde method. This reagent reacts with ammonia/ammonium and forms a fluorescent product. The fluorescence intensity (lex/em=360/450nm) is proportional to the ammonia concentration in the sample. Key Features: Fast and sensitive: Linear detection range of 0.012-1mM ammonia. Convenient and high-throughput: Homogeneous mix-incubate-measure type assay. Can be readily automated on HTS liquid handling systems for processing thousands of samples per day. Applications: Ammonia/ammonium determination in biological (e.g. urine) and environmental samples. Kit Components: A1383-56A: Reagent A, 1x1ml A1383-56B: Reagent B, 1x1ml A1383-56C: Assay Buffer, 1x20ml A1383-56D: Standard (NH4Cl), 1x400ul Storage and Stability: Store kit at -20C. Stable for 6 months after receipt.