Amphotericin B is a polyene antifungal agent for yeast, mold and mycoplasma. It was first isolated by Gold, et a., from Streptomyces nodosus in 1955. It is an amphoteric compound composed of a hydrophilic polyhydroxyl chain along one side and a lipophilic polyene hydrocarbon chain on the other. Amphotericin B is poorly soluble in water. Amphotericin B binds to sterols, preferentially to the primary fungal cell membrane sterol, ergosterol. This binding disrupts osmotic integrity of the fungal membrane, resulting in leakage of intracellular potassium, magnesium, sugars, and metabolites and then cellular death. Used in the preparation of baculoviral stocks: Amphotericin B Solution, 100X is composed of USP grade Amphotericin B in a High pH buffer (NaOH/Na+,H2O). The concentration of Amphotericin B in the final product is 0.25 g/L. Synonyms: Abelcet, Abelecet, AmBisome, Ampho-Moronal, Amphocin, Amphozone, Apothecon, Fungilin, Fungizona, Fungizone, Halizon, LNS-AmB, NS 718, NSC 527017 CAS No: 1397-89-3 Molecular Formula: C47H73NO17 Molecular Weight: 924.09 Appearance: Light yellow solution pH: 10-12 Activity: ~250ug/ml Sterility: Aseptically prepared by 0.2um filtration Working Concentration: Add aseptically 1ml concentrate to 100ml culture medium. Storage and Stability: May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for 6 months after receipt at -20C.
Molecular Weight:
924.09
Form:
Supplied as an easy to use 100X concentrate. Color is light yellow.
CAS Number:
[1397-89-3]
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