Control peptide for A2275-53B (antiserum) and A2275-53C (affinity purified antibody). The calcitonin family of bioactive peptides comprises of calcitonin, amylin, two calcitonin-gene related peptides (CGRP1 and CGRP2) and adrenomedullin (ADM). Calcitonin is 32aa peptide found in the parafollicular C cells of the thyroid in mammals. It is also found in a number of non-mammals. It regulated the mineral (calcium and phosphate) balance. Calcitonin causes hypercalcemia by acting as an inhibitor of osteoclast induced bone resorption. CGRP is a 37aa peptide produced by tissue specific processing of the calcitonin gene. Calcitonin is the major product in the thyroid. CGRP is the major product in neural tissues. CGRP is a potent cardiovascular agent. It has structural similarity with amylin. CGRP is found in two isoforms (CGRP-I and CGRP-II) that differs only by 3 amino acids. Amylin is a 37aa peptide produced in the pancreatic beta-cell secretory granules. It is co-released with insulin. Amylin also has CGRP-like effects on bone metabolism. Amylin has specific binding sites in the CNS. It it may regulate gastric emptying and influence carbohydrate metabolism. Adrenomedullin (ADM) is a 52aa hypotensive peptide. It has structural similarity with CGRP and amylin. ADM is produced in peripheral tissues, adrenal medulla, lung and kidney. ADM has specific receptors on astrocytes. It is unregulated in ischaemia. The calcitonin family peptides probably act through G-protein coupled membrane receptors. A homolog of calcitonin receptor, CRLR (calcitonin-receptor-like receptor human 461aa, rat/mouse 463aa) was identified. It is now shown that CRLR can function as either a CGRP receptor or an ADM receptor, depending upon which members of a new family of proteins called receptor activity modifying proteins (RAMP1-3) are expressed. Applications: Suitable for use in ELISA, Antibody Blocking. Not suitable for use in Western Blot due to low molecular weight. Other applications not tested. Recommended Dilution: Antibody Blocking: 5-10ug per 1ul A2275-53B (antiserum) or per 1ug A2275-53C (affinity purified antibody). ELISA: 1ug/ml Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4C for short-term only. For long-term storage, store at -20C. Aliquots are stable for at least 6 months at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Purity:
Highly purified
Form:
Supplied as a liquid in PBS, pH 7.2, 0.09% sodium azide.
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