Anti-Nuclear Antibodies (ANA) BioAssay(TM) ELISA Kit (Mouse)

Product Overview

• Article Name: Anti-Nuclear Antibodies (ANA) BioAssay(TM) ELISA Kit (Mouse)
• Biozol Catalog Number: USB-A2298-12J
• Supplier Catalog Number: A2298-12J
• Alternative Catalog Number: USB-A2298-12J-1
• Manufacturer: US Biological
• Category: Kits/Assays

Product Description

Principle of the Test: Anti-Nuclear Antibodies (ANA) ELISA kit is based on binding of mouse ANA IgG+IgA+IgM from serum samples to extracted nuclear antigen immobilized on microtiter wells. After a washing step, IgG (HRP) goat anti-mouse is added. After another washing step, to remove all the unbound enzyme conjugate, chromogenic substrate (TMB) is added and color developed. The enzymatic reaction (blue color) is directly proportional to the amount of ANA present in the sample. The reaction is terminated by adding stopping solution (converts blue to yellow). Absorbance is then measured on a microtiter well ELISA reader at 450nm. The activity of total mouse antibodies in samples is calculated relative to mouse ANA calibrators. Kit Components: A2298-12J1: Microtiter Strips, 1x96 wells A2298-12J2: Standard 50U/ml, 1x650ul A2298-12J3: Standard 200U/ml, 1x650ul A2298-12J4: Standard 500U/ml, 1x650ul A2298-12J5: Standard 1000U/ml, 1x650ul A2298-12J8: IgG (HRP) 100X, 1x150ul A2298-12J9: Sample Diluent 20X, 1x10ml A2298-12J10: Wash Solution 100X, 1x10ml A2298-12J11: TMB Substrate, 1x12ml A2298-12J12: Stop Solution (1% H2SO4), 1x12ml A2298-12J13: Low NSB Sample Diluent 1x30ml Storage and Stability: Store all components at 4C. Stable for 6 months after receipt. For maximum recovery of product, centrifuge the original vial prior to removing the cap.