Aurora B Serine/Threonine Kinase, Recombinant, Human full length (AURKB, STK12, Serine Threonine Kinase 12, Aurora B, AIK2, AIM1, AurB, IPL1, ARK2, Aurora Related Kinase 2)
Biozol Catalog Number:
USB-A4189-11
Supplier Catalog Number:
A4189-11
Alternative Catalog Number:
USB-A4189-11-10
Manufacturer:
US Biological
Category:
Molekularbiologie
Human Aurora kinase B (GenBank Accession No. NM_004217), (a.a. 2-344), expressed in an Baculovirus infected Sf9 cell expression system. Stability: 6 months at -80 C Purity: >95% by SDS-PAGE. Specific Activity: 22 U/ug. One unit is defined as the amount of enzyme that will phosphorylate 1 pmol of Ser/Thr 1 peptide substarte per minute at pH 7.4 and 30 C. Assay buffer: 50 mM HEPES, pH 7.4, 3 mM MgCl2, 3 mM MnCl2, 1 mM DTT, 3uM Na orthovanadate, 0.5 mM ATP, 4 uM aurora B substrate, and 0.2 ug Aurora B kinase. Application: useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.
