Assay by Immunoelectrophoresis resulted in a single precipitin arc against anti-avidin. Fluorchrome/Protein Ratio: ~2 moles Cy3.5/mole Avidin Absorption Wavelength: 581nm Emission Wavelength: 596nm Applications: Suitable for use in Immunofluorescence, FLISA (Fluorescent) and Western Blot. Other applications not tested. Recommended Dilutions: Immunofluorescence: 1:1000-1:5000 FLISA (Fluorescent): 1:10,000-1:50,000 Optimal dilutions to be determined by the researcher. Storage and Stability: Lyophilized powder may be stored at -20C. Stable for 12 months after receipt at -20C. Reconstitute with sterile ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20C. Reconstituted product is stable for 6 months at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Purity:
Purified by chromatography.
Form:
Supplied as a lyophilized powder in PBS, pH 7.2, 10mg/ml BSA (IgG and protease free), 0.01% sodium azide. Reconstitute with 1ml sterile dH2O. Labeled with Cy3.5.
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