Bacillus anthracis PA, Protective Antigen, Control Peptide (Anthrax)
Biozol Catalog Number:
USB-B0003-05N3
Supplier Catalog Number:
B0003-05N3
Alternative Catalog Number:
USB-B0003-05N3-50
Manufacturer:
US Biological
Category:
Molekularbiologie
Application:
ELISA, WB
Anthrax infection is initiated by the inhalation, ingestion, or cutaneous contact with Bacillus anthracis endospores. B. anthracis produces three polypeptides that comprise the anthrax toxin: protective antigen (PA), lethal factor (LF), and edema factor (EF) (1,2 for review). PA binds to two related proteins on the cell surface, these are termed tumor epithelial marker 8 (TEM8)/anthrax toxin receptor (ATR) (3) and capillary morphogenesis protein 2 (CMG2) (4), although it is still unclear which is physiologically relevant. Following PA binding to its receptor, PA is cleaved into two fragments by a furin-like protease. The bound fragment binds both LF and EF, the resulting complex is then endocytosed which allows the translocation of LF and EF into the cytoplasm (5). These toxins are usually sufficient to cause rapid cell death, and often the death of the organism. Location: 14aa near the carboxy-terminus of Anthrax protective antigen (1). Applications: The peptide is used for blocking the activity of Anthrax PA (CT) antibody. It usually blocks the antibody activity completely in Western blot by incubating the peptide with equal volume of antibody for 30 min at 37C. Storage and Stability: May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing.. Store at -20C. Aliquots are stable for 6 months after receipt at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Purity:
Purified
Form:
Supplied as a liquid in PBS, pH 7.2, 0.1% BSA, 0.02% sodium azide.
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