BAFF, Recombinant, Human (B Cell Activating Factor Belonging To The TNF Family, TNFSF13B, TNFSF20, TALL-1, BLyS, THANK, ZNTF4)

Product Overview

• Article Name: BAFF, Recombinant, Human (B Cell Activating Factor Belonging To The TNF Family, TNFSF13B, TNFSF20, TALL-1, BLyS, THANK, ZNTF4)
• Biozol Catalog Number: USB-B0003-66B
• Supplier Catalog Number: B0003-66B
• Alternative Catalog Number: USB-B0003-66B-20
• Manufacturer: US Biological
• Category: Molekularbiologie

Product Description

BAFF, a member of the TNF family of ligands, is expressed in T cells, macrophages, monocytes and dendritic cells. BAFF is involved in stimulation of B and T cell function, and is an important survival and maturation factor for peripheral B cells. BAFF signals through three different TNF receptors, TACI, BCMA and BAFFR. The human BAFF gene codes for a 285 amino acid type II transmembrane protein containing a 46 amino acid cytoplasmic domain, a 21 amino acid transmembrane domain, and a 218 amino acid extracellular domain. Recombinant human soluble BAFF is a 152 amino acid polypeptide (17kD), which contains the TNF-like portion of the extracellular domain of BAFF. Molecular Weight: ~17kD Biological Activity: Determined by a mouse splenocyte survival assay. The expected ED50 for this effect is 0.5-2ug/ml. Amino Acid Sequence: AVQGPEETVT QDCLQLIADS ETPTIQKGSY TFVPWLLSFK RGSALEEKEN KILVKETGYF FIYGQVLYTD KTYAMGHLIQ RKKVHVFGDE LSLVTLFRCI QNMPETLPNN SCYSAGIAKL EEGDELQLAI PRENAQISLD GDVTFFGALK LL Storage and Stability: Lyophilized and reconstituted products are stable for 6 months after receipt at -20C. Reconstitute with sterile ddH2O, 0.1% HSA or BSA. Aliquot to avoid repeated freezing and thawing. Store at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Product Properties

• Molecular Weight: 17
• NCBI: 006564
• UniProt: Q9Y275
• Purity: 95% by SDS-PAGE and HPLC. Endotoxin: 0.1ng/ug (1EU/ug)
• Form: Supplied as a lyophilized powder from 5mM Tris, pH 8.0, 3mM sodium chloride. Reconstitute with with sterile ddH2O, 0.1% HSA or BSA to 0.1-1mg/ml. Further dilutions can be made into other buffered solutions.