BCA-1/BLC, a CXC chemokine, is expressed in the liver, spleen, lymph nodes, appendix and stomach. It exerts its activities through its only receptor, CXCR5. BCA-1/BLC is a potent chemoattractant for B lymphocytes, and induces weak chemotactic response in T cells and macrophages. It manifests no activity on neutrophils and monocytes. Recombinant mouse BLC is a 9.5kD protein containing 88 amino acid residues, expressed in E. coli, including the four highly conserved cysteine residues present in CXC chemokines. Amino Acid Sequence: ILEAHYTNLK CRCSGVISTV VGLNIIDRIQ VTPPGNGCPK TEVVIWTKMK KVICVNPRAK WLQRLLRHVQ SKSLSSTPQA PVSKRRAA Biological Activity: Determined by its ability to chemoattract human B cells. Storage and Stability: Lyophilized and reconstituted products are stable for 6 months after receipt at -20C. Reconstitute with sterile ddH2O. For long term storage add 0.1% HSA or BSA. Aliquot to avoid repeated freezing and thawing. Store at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
98% (SDS-PAGE and HPLC). Endotoxin: 0.1ng/ug (1EU/ug).
Form:
Supplied as a lyophilized powder from a sterile-filtered solution without additives. Reconstitute with sterile ddH2O to a concentration of 0.1-1.0mg/ml.
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