BCMA IgG2a Fc, Fusion Protein (B Cell Maturation Protein)
Biozol Catalog Number:
USB-B0807-50B
Supplier Catalog Number:
B0807-50B
Alternative Catalog Number:
USB-B0807-50B-25
Manufacturer:
US Biological
Category:
Molekularbiologie
Application:
ELISA
The human B cell activating factor (BAFF) and APRIL (a proliferation inducing ligand) are both type II molecules belonging to the TNF superfamily. They are expressed by non-B cells and are down-regulated by mitogenic stimulation. BAFF and APRIL bind to at least two receptors: TACI (transmembrane activator and CAML-interactor) and BCMA (B cell maturation antigen), both of which are restricted to B cells. Ligation of these receptors with recombinant BAFF dramatically increases IgM production by peripheral blood B cells. Recently a third receptor for BAFF (BAFF-R) was described. BAFF and BAFF-R knockout mice have a reduced number of mature B cells in the periphery. However, TACI and BCMA knockouts do not share this phenotype, suggesting that BAFF-R may be the primary receptor for BAFF in mice. Cell surface BAFF can be cleaved to form a soluble trimeric molecule. Levels of soluble BAFF correspond with levels of autoantibodies in Sjogrens Syndrome. BCMA-muIg binds to recombinant BAFF-muCD8 and can inhibit binding of this molecule to receptors on Raji cells. BCMA IgG2a Fc, Fusion Protein is a soluble molecule consisting of the extracellular domain (54 amino acids) of human BCMA fused to murine IgG2a Fc (232 amino acids). Applications: Suitable for use in ELISA. BCMA-muIg binds to recombinant BAFF-muCD8 and can inhibit this molecules abiltiy to bind to receptors on Raji cells. Other applications not tested. Recommended Dilutions: Optimal dilutions to be determined by the researcher. Binding Inhibition of Raji Cell Receptors: B0807-50B was tested for its ability to block the binding of B0003-67EX to receptors on Raji cells. 5x10e5 human Raji cells were washed and incubated for 45 min. on ice with 80ul of C2432-01H at 1ug/ml. Cells were washed 2 times and incubated with C2259-11D. Cells were then washed 3 times, fixed and analyzed by FACS. Cells stained positive with a mean shift of 1.3 log10 fluorescent units when compared with a buffer control. This binding was 88% inhibited when B0003-67EX was pre-incubated with 50ug/ml B0807-50B. Storage and Stability: May be stored at 4C. For long-term storage, aliquot and store at 4C. Do not freeze. Aliquots are stable for at least 6 months. For maximum recovery of product, centrifuge the original vial prior to removing the cap. Further dilutions can be made in assay buffer.
Molecular Weight:
33
Purity:
Purified by Protein A affinity chromatography.
Form:
Supplied as a liquid in 50mM sodium phosphate, pH 7.5, 100mM potassium chloride ,150mM sodium chloride, 0.5mg/ml gentamicin sulfate.
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