The human B cell activating factor (BAFF) and APRIL(a proliferation inducing ligand) are both type II molecules belonging to the TNF superfamily. They are expressed by non-B cells, and are down-regulated by mitogenic stimulation.2 BAFF and APRIL bind to at least two receptors: TACI (transmembrane activator and CAML-interactor) and BCMA (B cell maturation antigen), both of which are restricted to B cells.3,4 Ligation of these receptors with recombinant BAFF dramatically increases IgM production by peripheral blood B cells 1. Recently a third receptor for BAFF (BAFF-R) was described.5 Cell surface BAFF can be cleaved to form a soluble trimeric molecule. 2BCMA-muIg binds to recombinant BAFF-muCD8 and can inhibit binding of this molecule to receptors on Raji cells. Applications: Suitable for use in ELISA. BCMA-muIg binds to recombinant BAFF-muCD8 and can inhibit this molecules abiltiy to bind to receptors on Raji cells. Other applications not tested. Recommended Dilutions: Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4C. For long-term storage, aliquot and store at 4C. Do not freeze. Aliquots are stable for at least 6 months. For maximum recovery of product, centrifuge the original vial prior to removing the cap. Further dilutions can be made in assay buffer.
Molecular Weight:
33
Purity:
Purified by Protein A affinity chromatography.
Form:
Supplied as a liquid in 50mM sodium phosphate, pH 7.5, 100mM potassium chloride ,150mM sodium chloride.
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