Control peptide for B0899-03N and B0899-03P. Antimicrobial peptides are a common mechanism of host defense utilized by a variety of species, from insects to humans. Defensins are a large family of broad-spectrum antimicrobial peptides, identified originally in leukocytes of rabbits and humans. Defensins, cationic/polar peptides (30-35aa, 3-4kD), are distinguished by a conserved tri-disulfides and a largely beta-Sheet structure. Defensins, expressed at the cell surface, have been hypothesized to function as a biochemical barrier against microbial infection by inhibiting colonization of the epithelium by wide range of pathogenic microorganisms. The genes encoding human alpha and beta defensins are clustered in a contiguous segment of chromosome 8p23. Defensins are classified into two families designated and based on distinctive although similar, tri-disulfide linkages in the peptides. beta-defensins are slightly larger and differ in the position and arrangement of 3 disulfides. In humans, six defensin (cryptidins), HD 1-6 (HD1-4 are also known as HNP1-4 for Human Neutrophil Peptides), and two defensins, HBD-1 and HBD-2, have been identified to date. Rat RBD-1 and RBD-2) and mouse (MBD1-4) homologues of the human beta-defensin have also been identified. alpha-defensins are encoded by genes designated DEFA1-6, whereas human bdefensins are encoded by the DEFB1 and DEFb2 genes. Source: Synthetic peptide corresponding to 17aa of human HBD-1, near the N-terminus. Molecular Weight: ~3kD Applications: Suitable for use in ELISA and Antibody Blocking. It is not suitable for use in Western. Other applications not tested. Recommended Dilution: ELISA: 1ug/ml and detected with antibodies Optimal dilutions to be determined by the researcher. Storage and Stability: Lyophilized powder may be stored at -20C. Stable for 12 months at -20C. Reconstitute with sterile buffer or ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Molecular Weight:
3
Purity:
Purified
Form:
Supplied as a lyophilized powder from PBS, pH7.5.
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