Defensins (alpha and beta) are cationic peptides with a broad spectrum of antimicrobial activity that comprise an important arm of the innate immune system. The a-defensins are distinguished from the b-defensins by the pairing of their three disulfide bonds. Four human b-defensins have been identified, BD-1, BD-2, BD-3 and BD-4. B-defensins are expressed on some leukocytes and at epithelial surfaces. In addition to their direct antimicrobial activities, they are chemoattractant towards immature dendritic cells and memory T cells. The B-defensin proteins are expressed as the C-terminal portion of precursors and are released by proteolytic cleavage of a signal sequence and, in the case of BD-1 (36aa), a propeptide region. B-defensins contain a six-cysteine motif that forms three intra-molecular disulfide bonds. B-Defensins are 3-5kD peptides ranging in size from 33-47 amino acid residues. Recombinant Human BD-1 is a 3.9kD protein containing 36 amino acid residues. Recombinant protein corresponding to 36aa from human BD1, expressed in E. coli Biological Activity: Determined by its ability to chemoattract CD34+ dendritic cells using a concentration range of 100-1000ng/ml. Amino Acid Sequence: DHYNCVSSGG QCLYSACPIFTKIQGTCYRG KAKCCK Storage and Stability: Lyophilized and reconstituted products are stable for 6 months after receipt at -20C. Reconstitute with sterile acetic acid. Aliquot to avoid repeated freezing and thawing. Store at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Molecular Weight:
3.9
Purity:
98% (SDS-PAGE and HPLC). Endotoxin: 0.1ng/ug (1EU/ug).
Form:
Supplied as a lyophilized powder from 10mM acetic acid. Reconstitute in 10mM acetic acid to a concentration of 0.1-1mg/ml. Do not vortex.
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