BfmI (SfeI)

Product Overview

• Article Name: BfmI (SfeI)
• Biozol Catalog Number: USB-B1086
• Supplier Catalog Number: B1086
• Alternative Catalog Number: USB-B1086-200,USB-B1086-1000
• Manufacturer: US Biological
• Category: Molekularbiologie

Product Description

Description: 5-C T R Y A G-3 3-G A Y R T C-5 Source: Bacillus firmus S8-336 Concentration: 10u/ul Unit Definition: One unit is defined as the amount of enzyme required to digest 1ug of lambda DNA in 1 hour at 37C in 50ul of assay buffer. Diluent Buffer: 10mM Tris-HCl, pH 7.4, 100mM KCl, 1mM EDTA, 1mM DTT, 0.2mg/ml BSA, 50% glycerol Storage Buffer: 10mM Tris-HCl, pH 7.4, 100mM KCl, 1mM DTT, 1mM EDTA, 0.2mg/ml BSA, 50% glycerol Supplied With: R1625: Restriction Enzyme Buffer A, 10X: Suplied as a liquid in 33mM Tris-acetate pH 7.9, 10mM magnesium acetate, 66mM potassium acetate, 0.1mg/ml BSA. Enzyme Properties: Stability during Prolonged Incubation: A minimum of 1.0unit of enzyme is required for complete digestion of 1ug of lambda DNA in 16 hours at 37C. Thermal Inactivation: Enzyme is inactivated by incubation at 65C for 20 minutes. Compatible Ends: C TGCAG-Alw44I Number of Recognition Sites in DNA: Lambda: 38 PhiX174: 6 pBR322: 4 pUC57: 4 pUC18/19: 4 pTZ19R/U: 6 M13mp18/19: 7 Quality Control: Overdigestion Assay: No detectable change in the specific fragmentation pattern is observed after 80-fold overdigestion (5u/ug lambda DNA x 16 hours) with BfmI (see Star Activity). Ligation/Recutting Assay: The ligation and recleavage assay was replaced with L0 test after validating experiments showed L0 test ability to trace nuclease and phosphatase activities with sensitivity that is higher than L/R by a factor of 100. Labeled Oligonucleotide (LO) Assay: No detectable degradation of a single-stranded and double-stranded labeled oligonucleotide was observed after incubation with 10units of restriction endonuclease for 4 hours. Storage and Stability: May be stored at 4C for short-term only. For long-term storage, store at -20C. Aliquots are stable for 6 months at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.