Biotin is used for mmunological purification in avidin/streptavidin-biotin binding mechanisms. Biotin, also known as vitamin H or B7, is a water-soluble B-complex vitamin which is composed of an ureido (tetrahydroimidizalone) ring fused with a tetrahydrothiophene ring. A valeric acid substituent is attached to one of the carbon atoms of the tetrahydrothiophene ring. Applications: Suitable for use in Western Blot, ELISA and Immunohistochemistry. Other applications not tested. Recommended Dilution: ELISA: 1:20,000- 1:200,000 Western Blot: 1:10,000- 1:40,000 Immunohistochemistry: 1:1000- 1:5000 Optimal dilutions to be determined by the researcher. Storage and Stability: Lyophilized powder may be stored at -20C. Stable for 12 months at -20C. Reconstitute with sterile ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20C. Reconstituted product is stable for 12 months at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Purity:
Purified by ion exchange chromatography. IEP Assay shows a single precipitin arc against source serum protein and IgG.
Form:
Supplied as a lyophilized powder from PBS, pH 7.2, 10mg/ml BSA (IgG and Protease free), 0.01% gentamicin sulfate. Labeled with horseradish peroxidase (HRP). Reconstitute with 5ml sterile ddH2O. Do not add sodium azide.
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