Bme1390I (ScrFI)

Product Overview

• Article Name: Bme1390I (ScrFI)
• Biozol Catalog Number: USB-B2180
• Supplier Catalog Number: B2180
• Alternative Catalog Number: USB-B2180-500
• Manufacturer: US Biological
• Category: Molekularbiologie

Product Description

5-C C N G G-3 3-G G N C C-5 Concentration: 10u/ul Source: Bacillus megaterium RFL1390 Buffer: 50mM Tris-HCl (pH 7.5), 10mM MgCl2, 100mM NaCl and 0.1mg/ml BSA. Incubate at 37C Diluent Buffer: 10mM Tris-HCl (pH 7.4 at 25C), 100mM KCl, 1mM EDTA, 1mM DTT, 0.2mg/ml BSA and 50% glycerol, or Storage Buffer. Storage Buffer: 10mM Tris-HCl (pH 7.5 at 25C), 50mM KCl, 1mM DTT, 0.1mM EDTA, 0.2mg/ml BSA and 50% glycerol. Overdigestion Assay: No detectable change in the specific fragmentation pattern is observed after 320-fold overdigestion (20u/ug DNA x 16 hours) with Bme1390I. Ligation/Recutting Assay: After 50-fold overdigestion (3u/ug DNA x 17 hours) with Bme1390I, more than 80% of the DNA fragments can be ligated in a reaction mixture containing 20-40u of T4 DNA ligase/1ug of fragments and 10% PEG at a 5-termini concentration of 1.8uM. More than 90% of these can be recut. Labeled Oligonucleotide (LO) Assay: No detectable degradation of a single-stranded and double-stranded labeled oligonucleotide was observed after incubation with 10units of restriction endonuclease for 4 hours. Methylation Effects: Bme1390I does not cut Cm5CNGG. Blocked by overlapping Dcm or CG methylation. Stability during Prolonged Incubation: A minimum of 0.2units of enzyme is required for complete digestion of 1ug of lambda DNA in 16 hours at 37C. Thermal Inactivation: Enzyme is inactivated by incubation at 80C for 20min. Compatible Ends: CC (C/G)GG-BcnI, SatI CC (A/T)GG-MvaI, SatI Number of Recognition Sites in DNA: Lambda: 184 PhiX174: 3 M13mp18/19: 11 pBR322: 16 pUC18/19: 12 pUC57: 12 pTZ19R/U: 10 pBluescriptIIKS(-/+): 11 pBluescriptIISK(-/+): 11 pACYC177: 14 pACYC184: 22 Unit Definition: One unit is defined as the amount of enzyme required to digest 1ug of lambda DNA dcm-in 1 hour at 37C in 50ul of assay buffer.