Brefeldin A (BFA) is a fungal metabolite demonstrated to reversibly interfere with anterograde transport from the endoplasmic reticulumto the Golgi apparatus (1,2). While initially isolated as an antibiotic (3), and does have a wide range of antibiotic activity, it is primarily used as a biological research tool for studying protein transport. Treatment leads to a rapid accumulation of proteins within the ER and collapse of the Golgi stacks. Treatment with BFA can also inhibit protein secretion (4) and prolonged exposure can induce apoptosis (5). The main target of BFA appears to be ADP-ribosylation factor (ARF), which is responsible for association of coat protein to the Golgi membrane (6,7). Synonyms: gamma-4-Dihydroxy-2-(6-hydroxy-1-heptenyl)-4-cyclopentanecrotonic acid lambda -lactone, Ascotoxin, BFA, Cyanein, Decumbin,g-4-Dihdyroxy-2-(6-hydroxy-1-heptenyl)-4-cyclopentanecrotonic Acid, sigma-Lactone, BFA, Cyanein, Decumbin, Synergisidin, Nectrolide CAS No: 20350-15-6 Molecular Formula: C16H24O4 Molecular Weight: 280.36 Appearance: Supplied as an off-white powder. Purity: 99% (HPLC) Solubility: Soluble in DMSO at 100mg/ml, soluble in ethanol at 25mg/ml with slight warming, very poorly soluble in water, maximum solubility in plain water is estimated to be about 50-100uM Storage and Stability: Lyophilized powder may be stored at -20C. Stable for 12 months at -20C. Reconstitute with DMSO or ethanol. Aliquot and store at -20C. Reconstituted product is stable for 6 months at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Molecular Weight:
280.36
Purity:
99% (HPLC)
Form:
Supplied as an off-white powder.
CAS Number:
[20350-15-6]
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