Selective medium for isolation of Salmonella. Description: Brilliant Green Agar was first described by Kristensen et al. in 1925 and later modified by Kauffman in 1933. It is classified by the USP as a highly selective medium for the recovery of Salmonella species except for typhoid and paratyphoid strains. Brilliant Green dye inhibits Gram positive and most Gram negative bacteria. Phenol red serves as a pH indicator for the fermentation of lactose or sucrose in the medium. Storage and Stability: Store powdered media at RT. Opened bottles should be capped tightly and kept in a dark, low humidity environment. Prepared media should be kept at 4C and used within a short period of time. Appearance: Light beige with a greenish tint, homogeneous, free flowing powder. Solubility: Brownish-green, slightly turbid. pH (25C): 6.90.2 Microbiological Analysis: Escherichia coli ATCC 25922: Inhibited Salmonella cholerasius ATCC 13076: Growth, Pink colonies Salmonella typhi ATCC 19430: Inhibited Salmonella typhimurium ATCC 14028: Growth, Pink colonies Staphylococcus aureus ATCC 25923: Inhibited Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.
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