5-Bromo-2-deoxyuridine (BrdU) is an analog of thymidine, and can be incorporated into the newly synthesized DNA of replicating cells (S-phase cells). BrdU is a commonly used in the detection of proliferating cells in living tissues. It works by substituting for thymidine during DNA replication and incorporating itself into the newly synthesized DNA (during S-phase). Antibodies specific for BrdU can then be used to detect the incorporated chemical, thus indicating cells that were actively replicating their DNA. Because BrdU can replace thymidine during the S phase of cell division, it can cause mutations. Bromodeoxyuridine (BrdU) is also used to prepare dNTP-BrdU solution for UV cross-linking of proteins to nucleic acids and to prepare complete MEM-10-Brdu or MEM-20-Brdu for vaccinia virus stock cultures. Synonyms: 5-Bromo-2-deoxyuridine, 5-BrdU, 2-Deoxy-5-bromorouridine, (+)-5-Bromo-1-[(2R,4S,5R)-4-hydroxy-5-(hydroxymethyl)oxolan-2-yl]pyrimidine-2,4-dione, Bromodeoxyuridine, Broxuridine, NSC 38297 CAS No: 59-14-3 Molecular Formula: C9H11BrN2O5 Molecular Weight: 307.10 Appearance: White to off white, crystalline or powder Purity (HPLC): 98% UV Content (on dry basis): 97% Ratio of Absorbances (pH2): 0.47-0.53 (A250/A260) 1.87-1.93 (A280/A260) 1.45-1.55 (A290/A260) Melting Point: ~187-189C Specific Rotation (C=1%, H2O): +23.0 Water (KF): 1% Solubility: Soluble in 0.1N NaOH and DMF, sparingly soluble in water, slightly soluble in ethanol and acetone, practically insoluble in ether RNase: None Detected DNase: None Detected Protease: None Detected Endotoxin: 0.5EU/mg
Molecular Weight:
307.1
CAS Number:
[59-14-3]
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