BseDI (SecI)

Product Overview

• Article Name: BseDI (SecI)
• Biozol Catalog Number: USB-B2905
• Supplier Catalog Number: B2905
• Alternative Catalog Number: USB-B2905-300
• Manufacturer: US Biological
• Category: Molekularbiologie

Product Description

5-C C N N G G-3 3-G G N N C C-5 Source: Bacillus stearothermophilus RFL1434 Concentration: 10u/ul Unit Definition: One unit is defined as the amount of enzyme required to digest 1ug of lambda DNA in 1 hour at 55C in 50ul of assay buffer. Dilution Buffer: 10mM Tris-HCl, pH 7.4 at RT, 100mM KCl, 1mM EDTA, 1mM DTT, 0.2mg/ml BSA, 50% glycerol. Storage Buffer: Supplied as a liquid in 10mM Tris-HCl, pH 7.5 at RT, 50mM KCl, 1mM DTT, 0.1mM EDTA, 0.2mg/ml BSA, 50% glycerol. Directions For Use: Incubate at 55C. (Incubation at 37C results in 10% activity.) Supplied with: R1625: Restriction Enzyme Buffer A, 10X. Suitable for use in Double Digest assays. Enzyme Properties: Thermal Inactivation: Enzyme is inactivated by incubation at 80C for 20min. Stability during Prolonged Incubation: A minimum of 0.2units of enzyme is required for complete digestion of 1ug of lambda DNA in 16 hours at 55C Number of Recognition Sites in DNA: Lambda: 105 PhiX174: 6 M13mp18/19: 9 pBR322: 8 pUC18/19: 5 pUC57: 4 pTZ19R/U: 5 Methylation Effects: DAM: Never overlaps - no effect Dcm: May overlap - no effect CpG: May overlap - no effect EcoKI: Never overlaps - no effect EcoBI: Never overlaps - no effect Quality Control: Overdigestion Assay: No detectable change in the specific fragmentation pattern is observed after 160-fold overdigestion (10u/ug lambda DNA x 16 hours) with BseDI. Ligation/Recutting Assay: After 50-fold overdigestion (3u/ug DNA x The Ligation/Recutting assay was replaced with LO test after validating experiements showed LO test ability to trace nuclease and phophatase activities with sensitivity that is higher than L/R by a factor of 10. Labeled Oligonucleotide (LO) Assay: No detectable degradation of a single-stranded and double-stranded labeled oligonucleotide was observed after incubation with 10 units of BseDI for 4 hours. Storage and Stability: May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing.. Store at -20C. Aliquots are stable for 6 months after receipt at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. .