Bsh1236I (FnuDII)

Product Overview

• Article Name: Bsh1236I (FnuDII)
• Biozol Catalog Number: USB-B2922
• Supplier Catalog Number: B2922
• Alternative Catalog Number: USB-B2922-500,USB-B2922-2500
• Manufacturer: US Biological
• Category: Molekularbiologie

Product Description

5-C GC G-3 3-G CG C-5 Source: Bacillus sphaericus RFL1236 Concentration: 10u/ul Incubation Temperature: 37C Enzyme Properties: Methylation Effect: CpG : completely overlaps, blocked Dam, Dcm, EcoKl, EcoBl: never overlaps, no effect Stability during Prolonged Incubation: A minimum of 0.1units of Bsh1236I is required for complete digestion of 1ug of lambda DNA in 16 hours at 37C. Thermal Inactivation: Bsh1236I is inactivated by incubation at 65C for 20 minutes. Quality Control: Overdigestion Assay: No detectable change in the specific fragmentation pattern is observed after 160-fold overdigestion (10u/ug lambda DNA x 16 hours) with Bsh1236I. Ligation/Recutting Assay: The ligation and recleavage assay was replaced with L0 test after validating experiments showed L0 test ability to trace nuclease and phosphatase activities with sensitivity that is higher than the ligation/recutting test by a factor of 100. Labeled Oligonucleotide (LO) Assay: No detectable degradation of a single-stranded and double-stranded labeled oligonucleotide was observed after incubation with 10units of Bsh1236I for 4 hours. Number of Recognition Sites in DNA: Lambda: 157 PhiX174: 14 M13mp18/19: 18 pBR322: 23 pUC18/19: 10 pUC57: 11 pTZ19R/U: 11 Protocol for Digestion: Add: Nuclease free water: 16ul R1625-04: 2ul DNA (0.5-1ug/ml): 1ul B2922: 0.5-2ul Mix gently and spin down for a few seconds. Incubate at 37C for 1-16 hours Protocol for Digestion Directly after Amplification: Add: PCR Reaction Mixture: 10ul (~0.1-0.5ug of DNA) Nuclease free water: 18ul R1625-04: 2ul B2922: 1-2ul Mix gently and spin down for a few seconds. Incubate at 37C for 1-16 hours. Storage Buffer: Supplied as a liquid in 10mM Tris-HCl, pH 7.5, 50mM KCl, 1mM DTT, 0.1mM EDTA, 0.2mg/ml BSA, 50% glycerol. Diluent Buffer: 10mM Tris-HCl, pH 7.4, 100mM KCl, 1mM EDTA, 1mM DTT, 0.2mg/ml BSA and 50% glycerol. Supplied with: R1625: Restriction Enzyme Buffer A, 10X: Dilute to 1X for use. 1X buffer composition is 33mM Tris-acetate, pH 7.9, 10mM magnesium acetate, 66mM potassium acetate, 0.1mg/ml BSA R1625-04: Restriction Enzyme Buffer E, 10X: Dilute to 1X for use. 1X buffer composition is 10mM Tris-HCl, pH 8.5 at 37C, 10mM MgCl2, 100mM KCl, 0.1mg/ml BSA Storage and Stability: Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for 6 months after receipt at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.