5-G G G C C C-3 3-C C C G G G-5 Unlike ApaI, Bsp120I produces DNA fragments with a 4-base 5-extension. Concentration: 10u/ul, 50u/ul(HC) Source: Bacillus subtilis RFL120 Buffer: 10mM Tris-HCl (pH 7.5), 10mM MgCl2 and 0.1mg/ml BSA. Incubate at 37C. Diluent Buffer: 10mM Tris-HCl (pH 7.4 at 25C), 100mM KCl, 1mM EDTA, 1mM DTT, 0.2mg/ml BSA and 50% glycerol. Storage Buffer: 10mM Tris-HCl (pH 7.4 at 25C), 100mM KCl, 1mM DTT, 1mM EDTA, 0.2mg/ml BSA and 50% glycerol.
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