Bst1107I (SnaI)

Product Overview

• Article Name: Bst1107I (SnaI)
• Biozol Catalog Number: USB-B3000-10
• Supplier Catalog Number: B3000-10
• Alternative Catalog Number: USB-B3000-10-500
• Manufacturer: US Biological
• Category: Molekularbiologie

Product Description

Sequence: 5-G T A T A C-3 3-C A T A T G-5 Source: Bacillus stearothermophilus RFL1107 Concentration: 10u/ul Unit Definition: One unit is defined as the amount of Bst1107I is required to digest 1ug of lambda DNA in 1 hour at 37C in 50ul of recommended reaction buffer. Form: Supplied as a liquid in 10mM Tris-HCl pH 7.4, 100mM KCl, 1mM DTT, 1mM EDTA, 0.2mg/ml BSA, 50% glycerol. Supplied with: R1625: Restriction Enzyme Buffer A, 10X: Dilute to 1X for use. 1X buffer composition is 33mM Tris-acetate pH 7.9 at 37C, 10mM magnesium acetate, 66mM potassium acetate, 0.1mg/ml BSA. R1625-03: Restriction Enzyme Buffer D, 10X: Dilute to 1X for use. 1X buffer composition is 50mM Tris-HCl, pH 7.5, 10mM MgCl2, 100mM sodium chloride, 0.1mg/ml BSA. Thermal Inactivation: Enzyme is inactivated by incubation at 80C for 20 minutes. Diluent Buffer: 10mM Tris-HCl pH 7.4, 100mM KCl, 1mM DTT, 1mM EDTA, 0.2mg/ml BSA, 50% glycerol. Enzyme Properties: Methylation Effects: Dam, Never overlaps - no effect Dcm: Never overlaps - no effect CpG: May overlap - cleavage impaired EcoKI: Never overlaps - no effect EcoBI: Never overlaps - no effect Stability during Prolonged Incubation: A minimum of 0.1units of Bst1107I is required for complete digestion of 1ug of lambda DNA in 16 hours at 37C. Digestion of Agarose-embedded DNA: A minimum of 5 units is required for complete digestion of 1ug of agarose-embedded lambda DNA in 16 hours. Number of Recognition Sites in DNA: Lambda: 3 PhiX174: 0 M13mp18/19: 0 pBR322: 1 pUC18/19: 0 pUC57: 0 pTZ19R/U: 0 Overdigestion Assay: No detectable change in the specific fragmentation pattern is observed after a 160-fold overdigestion with Bst1107I (10U/ug lambda DNA x 16 hours). Ligation and Recleavage (L/R) Assay: The ligation and recleavage assay was replaced with LO test after validating experiments showed LO test ability to trace nuclease and phosphatase activities with sensitivity that is higher than L/R by a factor of 100. Labeled Oligonucleotide (LO) Assay: No detectable degradation of single-stranded or double- stranded labeled oligonucleotides occurred during incubation with 10 units of Bst1107I for 4 hours. Blue/White Cloning Assay: The B/W assay was replaced with L0 test after validating experiments showed L0 test ability to detect nuclease and phophatase activities with sensitivity that equals to that of B/W test. Storage and Stability: May be stored at 4C. For long-term storage, aliquot and store at -20C. Aliquots are stable for 6 months after receipt. For maximum recovery of product, centrifuge the original vial prior to removing the cap. Further dilutions can be made in assay buffer.