BstXI

Product Overview

• Article Name: BstXI
• Biozol Catalog Number: USB-B3001
• Supplier Catalog Number: B3001
• Alternative Catalog Number: USB-B3001-500,USB-B3001-2500
• Manufacturer: US Biological
• Category: Molekularbiologie

Product Description

5-C C A N N N N NN T G G-3 3-G G T NN N N N N A C C-5 Source: Bacillus stearothermophilus X Concentration: ~10u/ul Unit Definition: One unit is defined as the amount of BstXI required to digest 1ug of lambda DNA in 1 hour at 55C in 50ul of assay buffer. Supplied With: R1625: Restriction Enzyme Buffer A, 10X: Dilute to 1X for use. 1X buffer composition is 33mM Tris-acetate pH 7.9, 10mM magnesium acetate, 66mM potassium acetate, 0.1mg/ml BSA. R1625-03: Restriction Enzyme Buffer D, 10X: Dilute to 1X for use. 1X buffer composition is 50mM Tris-HCl, pH 7.5, 10mM MgCl2, 100mM sodium chloride, 0.1mg/ml BSA. Diluent Buffer: 10mM Tris-HCl, pH 7.4, 100mM KCl, 1mM EDTA, 1mM DTT, 0.2mg/ml BSA and 50% glycerol. Storage Buffer: 10mM Tris-HCl, pH 7.5, 50mM KCl, 1mM DTT, 0.1mM EDTA, 0.2mg/ml BSA and 50% glycerol. Incubation Temperature: 55C (Incubation at 37C results in 50% activity) Stability During Prolonged Incubation: A minimum of 0.1 units of BstXI is required for complete digestion of 1ug of lambda DNA in 16 hours at 55C. Overdigestion Assay: No detectable change in the specific fragmentation pattern is observed after 15-fold overdigestion (15u/ug lambda DNA x 1 hours) (see Star Activity) Ligation/Recutting Assay: The ligation and recleavage assay was replaced with L0 test after validating experiments showed L0 test ability to trace nuclease and phosphatase activities with sensitivity that is higher than L/R by a factor of 100. Labeled Oligonucleotide (LO) Assay: No detectable degradation of a single-stranded or double-stranded labeled oligonucleotide was observed after incubation with 10 units of BstXI for 4 hours. Blue/White Cloning Assay: The B/W assay was replaced by the L0 test after validating experiments showed L0 test ability to trace nuclease and phosphatase activities with sensitivity that equals to that of B/W test. Methylation Effects: Dam: never overlaps- no effect Dcm: may overlap- cleavage impaired CpG: never overlaps- no effect EcpKl: never overlaps- no effect EcoBl: never overlaps- no effect Star Activity: Excess of BstXI (20U/ug DNA x 1 hour) may result in star activity. Thermal Inactivation: BstXI is inactivated by incubation at 80C for 20 minutes. Digestion of Agarose-embedded DNA: A minimum of 5 units of BstXI is required for complete digestion of 1ug of agarose-embedded lambda DNA in 16 hours. Number of Recognition Sites in DNA: Lambda: 13 PhiX174: 3 pBR322: 0 pUC57: 0 pUC18/19: 0 pTZ19R/U: 0 M13mp18/19: 0 Storage and Stability: Aliquot and store at -20C. Aliquots are stable for 6 months after receipt at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.