CCL22/MDC is a small cytokine belonging to the CC chemokine family. This protein displays chemotactic activity for natural killer cells, chronically activated T lymphocytes, monocytes and dendritic cells. It interacts with cell surface chemokine receptors CCR4. This chemokine may play a role in the trafficking of activated T lymphocytes to inflammatory sites and other aspects of activated T lymphocyte physiology. Recombinant human CCL22/MDC, fused to His-tag at N-terminus, was expressed as insoluble protein aggregate in , expressed in E. coli and purified by conventional chromatography, after refolding of the isolated inclusion bodies in a renaturation buffer. Source: Recombinant human CCL22/MDC, aa25-93, His-tagged, expressed in E. coli AA Sequence: MGSSHHHHHH SSGLVPRGSH MGPYGANMED SVCCRDYVRY RLPLRVVKHF YWTSDSCPRP GVVLLTFRDK EICADPRVPW VKMILNKLSQ Applications: Suitable for use in ELISA and Western Blot. Other applications not tested. Recommended Dilution: Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4C for short-term only. For long-term storage, aliquot and store at -20C. Aliquots are stable for at least 6 months at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
