CaiI (AlwNI)

Product Overview

• Article Name: CaiI (AlwNI)
• Biozol Catalog Number: USB-C0112
• Supplier Catalog Number: C0112
• Alternative Catalog Number: USB-C0112-500
• Manufacturer: US Biological
• Category: Molekularbiologie

Product Description

5-C A G N N N C T G-3 3-G T C N N N G A C-5 Concentration: 10u/ul Source: Comamonas acidovorans Lti 19-021 Buffer: 33mM Tris-acetate (pH 7.9), 10mM magnesium acetate, 66mM potassium acetate and 0.1mg/ml BSA. Incubate at 37C. Diluent Buffer: 10mM Tris-HCl (pH 7.4 at 25C), 100mM KCl, 1mM EDTA, 1mM DTT, 0.2mg/ml BSA and 50% glycerol. Storage Buffer: 10mM Tris-HCl (pH 7.4 at 25C), 100mM KCl, 1mM DTT, 1mM EDTA, 0.2mg/ml BSA and 50% glycerol. Overdigestion Assay: No detectable change in the specific fragmentation pattern is observed after 320-fold overdigestion (20u/ug lambda DNA x 16 hours) with CaiI. Ligation/Recutting Assay: After 50-fold overdigestion (3u/ug DNA x 17 hours) with CaiI, more than 95% of the DNA fragments can be ligated at a 5-termini concentration of 0.3uM. More than 95% of these can be recut. Labeled Oligonucleotide (LO) Assay: No detectable degradation of a single-stranded and double-stranded labeled oligonucleotide was observed after incubation with 10units of restriction endonuclease for 4 hours. Methylation Effects: CaiI does not cut CAGNNCm5CTG. Blocked by overlapping Dcm methylation. Stability during Prolonged Incubation: A minimum of 0.2units of enzyme is required for complete digestion of 1ug of lambda DNA in 16 hours at 37C. Thermal Inactivation: Enzyme is inactivated by incubation at 65C for 20min. Digestion of Agarose-embedded DNA: A minimum of 5units of enzyme is required for digestion of 1ug of agarose-embedded lambda DNA in 16 hours. Number of Recognition Sites in DNA: Lambda: 41 PhiX174: 0 M13mp18/19: 1 pBR322: 1 pUC18/19: 1 pUC57: 1 pTZ19R/U: 1 pBluescriptIIKS(-/+): 1 pBluescriptIISK(-/+): 1 pACYC177: 1 pACYC184: 3 Unit Definition: One unit is defined as the amount of enzyme required to digest 1ug of lambda DNA in 1 hour at 37C in 50ul of assay buffer.