Calmodulin is a ubiquitous Ca2+-binding protein that serves as a physiological effector of a wide range of biological processes. Purified from bovine brain. Calmodulin (CaM) is a ubiquitous Ca2+-binding protein distributed in eukaryotic cells that serves as a major physiological effector for a wide range of cellular responses. The Ca2+-calmodulin complex is involved in the maintenance of intracellular Ca2+ homeostasis, as well as in the regulation of the intracellular concentration of other second messengers, such as cyclic AMP and inositol trisphosphate. Mutations in the CaM sequence can result in diverse and potentially life-threatening pathologies such as ventricular tachycardia, congenital arrhythmia, and long QT syndrome. These mutations have the capacity to modify CaM's ability to bind to Ca2+ and its target proteins and peptides, thereby disrupting their subsequent activity. Ca2+/CaM complex and CaM-binding domains (CaMBDs) interactions influence memory formation, muscle contraction, cellular metabolism, and cytoskeletal rearrangements. Source: Bovine Brain Specific Activity: 250,000 units/mg protein Unit Definition: One unit is defined as the amount of calmodulin that gives rise to 50% of the maximal enzyme activation of a standard level of activator-deficient calcineurin. Applications: Suitable for use in Cell based assays. Other applications not tested. Recommended Dilution: Optimal dilutions to be determined by the researcher. Storage and Stability: Lyophilized and reconstituted products are stable for 6 months after receipt at -20C. Reconstitute with sterile ddH2O or aqueous buffer. Aliquot to avoid repeated freezing and thawing. Store at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Purity:
95% (SDS-PAGE)
Form:
Supplied as a lyophilized powder in 30mM HEPES, 1mM CaCl2, 0.1mM DTT, pH 7.4. Reconstitute with sterile ddH2O or aqueous buffer.
CAS Number:
[73298-54-1]
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