Pure Calystega sepiem Lectin (Calsepa) from Hedge Bindweed Rhizomes, Alkaline Phosphatase conjugated. Lectins are derived from the extracts of plants, animals, viruses and microorganisms and are known to agglutinate red blood cells. These agglutinins can select cell types according to blood group activities utilizing sugar-binding mechanisms. Lectins form precipitates with glycoconjugates and are useful for identifying or separating oligosaccharides with identical sugar compositions such as galactose, mannose or glucose. Carbohydrate Specificity: Maltose=Mannose>>Glucose Inhibitory Carbohydrate: Methyl alpha-mannopyranoside (best inhibitor) > methyl alpha-glucoside > alpha-glucosides or alpha-glucoside modified at 2-position. Activity: Calsepa will agglutinate untreated and trypsin-treated rabbit and human type A erythrocytes. Chemical Used for Conjugation: Alkaline Phosphatase. Storage and Stability: Store product at 4C. DO NOT FREEZE! Stable at 4C for 6 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. For maximum recovery of product, centrifuge the original vial prior to removing the cap.
Purity:
Purified
Form:
Supplied as a liquid in 0.01M PBS, 0.15M sodium chloride, pH 7.2-7.4, 20-50% glycerol, 0.05% sodium azide. Labeled with Alkaline Phosphatase (AP).
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