| Calyculin A, a polyene phosphate, was initially isolated from the marine sponge Discodermia calyx. It is a very potent, cell-permeable PP-1 and PP-2 inhibitor (IC50: 2nM and 0.5-1nM respectively, and extremely specific for PP-1 and PP2A classes of protein serine/threonine phosphatase among other acidic, alkaline and protein phosphatases. Calyculin A is a polyene phosphate, a potent inhibitor (Ki ~ 0.1nM) with high specificity for the PP-1 and PP-2 classes of protein serine/threonine phosphatase. Synonyms: (-)-Calyculin A, N-[(3S)-[4-(1E)-3-[(2R,3R,4R,7S,8S,9R)-2-[(1S,3S,4S,5R,7E,9E,11E,13Z)-14-Cyano-3,5-dihydroxy-1-methoxy-4,6,8,9,13-pentamethyl-7,9,11,13-tetradecatetraenyl]-9-hydroxy-4,4,8-trimethyl-3-(phosphonooxy)-1,6-dioxaspiro[4.5]dec-7-yl]-1-propenyl]-2-oxazoly]butyl]-4-deoxy-4-(dimethylamino)-5-O-methyl-L-ribonamide, [(2R,3R,5R,7R,8S,9S)-2-[(1S,3S,4S,5R,6R,7E,9E,11E,13Z)-14-cyano-3, 5-dihydroxy-1-methoxy-4,6,8,9,13-pentamethyltetradeca-7,9,11, 13-tetraenyl]-9-[(E)-3-[2-[(2S)-4-[[(2S,3S,4S)-4-(dimethylamino)-2, 3-dihydroxy-5-methoxypentanoyl]amino]butan-2-yl]-1, 3-oxazol-4-yl]prop-2-enyl]-7-hydroxy-4,4,8-trimethyl-1, 10-dioxaspiro[4.5]decan-3-yl] dihydrogen phosphate CAS No: 101932-71-2 Molecular Formula: C50H81N4O15P Molecular Weight: 1009.17 Appearance: Calyculin A is supplied as a lyophilized clear film. For 10uM stock, reconstitute in 1ml DMSO. Store in aliquots at -20C in the dark. Treat cells with 50-100nM calyculin A for 5-30 minutes. Store in aliquots tightly sealed (unopened) at -20C in the dark. Purity: 98% (HPLC) Melting Point: 247-249C Solubility: Soluble in DMSO and EtOH, insoluble in water. Calyculin A must first be dissolved in an organic solvent such as DMSO, ethanol, acetone or methanol. The recommended solvent for stock solutions to be used for biological experiments is DMSO. Storage and Stability: Lyophilized powder may be stored at -20C. Stable for 12 months at -20C. Reconstitute with DMSO and EtOH. Aliquot to avoid repeated freezing and thawing. Store at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. Do not expose to sunlight or fluorescent lights for extended periods. Warning! This product is a suspect cancer hazard and tumor promoter. Gloves and mask should be worn when using this compound. Care must be taken to prevent contact through all routes of exposure. This compound is sold only for use in extremely dilute solutions for biological research. No other use is intended and any other use involves substantial hazards. This compound should never be handled in powder or aerosol form or in any other form susceptible to uncontrolled release in the laboratory, even in very small quantities. |
