Canavalia ensiformis (Concanavalin A, Jackbean) (Agarose)
Biozol Catalog Number:
USB-C1045-10A
Supplier Catalog Number:
C1045-10A
Alternative Catalog Number:
USB-C1045-10A-5
Manufacturer:
US Biological
Category:
Molekularbiologie
Purified Canavalia ensiformis (Con A) covalently linked to agarose beads (exclusion limit MW 5-15 million). Lectins are derived from the extracts of plants, animals, viruses and microorganisms and are known to agglutinate red blood cells. These agglutinins can select cell types according to blood group activities utilizing sugar-binding mechanisms. Lectins form precipitates with glycoconjugates and are useful for identifying or separating oligosaccharides with identical sugar compositions such as galactose, mannose or glucose. Bead Size: 50-250 microns Spacer: None Linkage: Imidoester Carbohydrate for Elution: 0.2M Mannose or Methyl alpha-D-Mannopyranoside in Buffer. Note: Calcium is REQUIRED for binding. 0.5mM Calcium is the maximum concentration in buffer that will not form a white precipitate. Storage and Stability: May be stored at 4C. For long-term storage, aliquot and store at 4C. Do not freeze. Aliquots are stable for 6 months. For maximum recovery of product, centrifuge the original vial prior to removing the cap. Further dilutions can be made in assay buffer.
Purity:
Purified
Form:
Supplied as a suspension in 0.05M Tris, 0.15M sodium chloride, 0.004M calcium chloride, pH 7.0, 0.05% sodium azide. Covalently linked to agarose beads (exclusion limit MW 5-15 million).
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