Canavalia ensiformis, Succinyl (Concanavalin A, Jackbean) (FITC)
Biozol Catalog Number:
USB-C1045-45
Supplier Catalog Number:
C1045-45
Alternative Catalog Number:
USB-C1045-45-5
Manufacturer:
US Biological
Category:
Molekularbiologie
Pure Canavalia ensiformis, succinyl lectin (Con A) from Jackbean, conjugated to FITC. Lectins are derived from the extracts of plants, animals, viruses and microorganisms and are known to agglutinate red blood cells. These agglutinins can select cell types according to blood group activities utilizing sugar-binding mechanisms. Lectins form precipitates with glycoconjugates and are useful for identifying or separating oligosaccharides with identical sugar compositions such as galactose, mannose or glucose. Carbohydrate Specificity: alpha-D-Mannose, alpha-D-Glucose, Branched mannose. Inhibitory Carbohydrate: Methyl alpha-D-Mannopyranoside >> alpha-D-Mannose>> alpha-D-Glucose. Activity: Con A is a relatively weak blood agglutinin More than 10ug/ml may be required to give visible agglutination of neuraminidase treated human erythrocytes. Chemical Used for Conjugation: FITC Storage and Stability: May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for 6 months at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. FITC conjugates are sensitive to light.
Form:
Supplied as a liquid in 0.05M Tris, 0.15M sodium chloride, 0.004M calcium chloride, pH 7.0, 0.05% sodium azide. Labeled with FITC.
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