16-aa peptide sequence mapping near the C-terminus of mouse CPT-2L (KLH coupled). Species Sequence Homology: rat (93%), human (86%). No significant sequence homology is seen with CPT-1 or any other protein.
In fatty acid synthesis, catalytic formation of malonyl-CoA (precursor for long-chain fatty acyl-CoA, LCFA-CoA) from acetyl-CoA by Acetyl-CoA carboxylase (ACC-1) is the rate limiting step. The translocation of LCFA-CoA from cytosol to mitochondria is catalyzed by two carnitine palmitoyl transferases (CPT-1 & CPT-2) and regulated by ACC-2, the rate limiting step of mitochondrial fatty acid b-oxidation. Activities of ACC-1 and 2 are regulated by their phosphorylation by 5-AMP-activated protein kinase (AMPK). Diabetes deranges AMPK master-switch and represses the ACC-1 gene-expression and stimulates excessive fatty acid oxidation which in turn interferes with glucose metabolism. Mitochondrial oxidation of LC-FCA is initiated by the sequential action of CPT-1, which is located in the outer membrane, and CPT-2, which is located in the inner membrane together with a carnitine-acylcarnitine translocase. CPT-2 (mouse/rat/human 658-aa, ~74kD, chromosome 1p32, ~20% identity with CPT1) is a ubiquitous malonyl-CoA-insensitive transferase localized in the inner mitochondrial membrane. It catalyzes the re-synthesis of acyl-CoA from acyl-carnitines. CPT-2 deficiency leads to the most commonly inherited, lipid myopathy in adults characterized by exercise-induced pain, stiffness, and myoglobinuria. The aa sequences of the two isoforms are ~61% identical. Applications: Suitable for use in ELISA, Western Blot. Other applications not tested. Recommended Dilution: Western Blot: 1-10ug/ml ELISA: 1:10,000-1:100,000 using 50-100ng of control peptide/well. Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4C for short-term only. For long-term storage, aliquot and store at -20C. Aliquots are stable for at least 12 months at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Purity:
Purified by immunoaffinity chromatography.
Form:
Supplied as a liquid in PBS, pH 7.4 and 0.09% sodium azide, 40% glycerol.
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