Cartilage Intermediate-Layer Protein 1 (CILP-1), Sheep
Biozol Catalog Number:
USB-C1394-50
Supplier Catalog Number:
C1394-50
Alternative Catalog Number:
USB-C1394-50-100
Manufacturer:
US Biological
Host:
Sheep
Category:
Antikörper
Application:
ELISA, WB
Immunogen:
Recombinant protein corresponding to Arg724-Asn1184 from human CILP-1, expressed in NS0 cells.
The CILP-1 (cartilage intermediate-layer protein 1) gene product is a monomeric glycoprotein precursor of two secreted, proteolytically generated products, a 90kD N-terminal CILP-1, and a 62kD C-terminal NTPPHase-homolog. It is found in both hyaline and fibrocartilage. Although the C-terminal NTPPHase-homolog (aa 725-1184) does not show enzyme activity, it may participate in matrix structural integrity. Human CILP-1 C-terminus shares 93% and 94% aa identity with porcine and mouse CILP-1 C-terminus, respectively. Applications: Suitable for use in Direct ELISA and Western Blot. Other applications not tested. Recommended Dilution: Western Blot: 0.1ug/ml with recombinant human CILP-1 C-Terminal Fragment Optimal dilutions to be determined by the researcher. Storage and Stability: Lyophilized and reconstituted products are stable for 12 months after receipt at -20C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
